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Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome
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Neurosciences
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Journal JoVE Neurosciences
Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome

Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome

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08:49 min

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September 23, 2015

DOI:

10.3791/53165-v

DOI:
10.3791/53165-v

•

08:49 min
September 23, 2015

•

1 Views
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1Ross University School of Veterinary Medicine, 2Charles E. Schmidt College of Medicine,Florida Atlantic University

Summary

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This protocol describes a rapid and simplified in situ hybridization method ideal forparaformaldehyde-prefixed brain, thus reducing the need for prolonged complex steps while using fresh frozen tissues. The method is validated using the identification of the serotonin 5-HT2A receptor gene htr2a in rats.

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In Situ HybridizationOligonucleotide ProbesParaformaldehyde-fixed BrainSerotonin5-HT2A ReceptorMDMASerotonin Syndrome

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Cite this Article

Shokry, I. M., Callanan, J. J., Sousa, J., Tao, R. Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome. J. Vis. Exp. (103), e53165, doi:10.3791/53165 (2015).

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