JoVE Journal
Neuroscience
Neuroscience
需要订阅 JoVE 才能查看此 内容. 登录或开始免费试用。
章节
总结概括
This protocol describes a rapid and simplified in situ hybridization method ideal forparaformaldehyde-prefixed brain, thus reducing the need for prolonged complex steps while using fresh frozen tissues. The method is validated using the identification of the serotonin 5-HT2A receptor gene htr2a in rats.