Journal
/
神经科学
/
Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome

Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome

8,704 Views

•

08:49 min

•

September 23, 2015

DOI:

10.3791/53165-v

DOI:
10.3791/53165-v

•

08:49 min
September 23, 2015

•

1 Views
, , ,
1Ross University School of Veterinary Medicine, 2Charles E. Schmidt College of Medicine,Florida Atlantic University

Summary

Automatically generated

This protocol describes a rapid and simplified in situ hybridization method ideal forparaformaldehyde-prefixed brain, thus reducing the need for prolonged complex steps while using fresh frozen tissues. The method is validated using the identification of the serotonin 5-HT2A receptor gene htr2a in rats.

Explore More Videos

In Situ HybridizationOligonucleotide ProbesParaformaldehyde-fixed BrainSerotonin5-HT2A ReceptorMDMASerotonin Syndrome

Read Article

Cite this Article

Shokry, I. M., Callanan, J. J., Sousa, J., Tao, R. Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome. J. Vis. Exp. (103), e53165, doi:10.3791/53165 (2015).

Download .ris file

Copy

Share Video

.

Copy