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고해상도를 사용하여 뇌 섹션에서 Axonally 지역화 된 mRNA를 검출<em> 현장에서</em> 하이브리드
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Neurosciences
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Journal JoVE Neurosciences
Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
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고해상도를 사용하여 뇌 섹션에서 Axonally 지역화 된 mRNA를 검출<em> 현장에서</em> 하이브리드

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11:24 min

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June 17, 2015

DOI:

10.3791/52799-v

DOI:
10.3791/52799-v

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11:24 min
June 17, 2015

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3 Views
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1College of Physicians and Surgeons,Columbia University

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Axonally Localized MRNAsIn Situ HybridizationRNAscopeAxonal CounterstainFluorescence ImmunohistochemistryHistological DyesAtf4 MRNATranscriptome AnalysisVertebrate AxonsAxon PathfindingAxon BranchingNeurodegenerationSingle-molecule DetectionSubcellular LocalizationMature Mouse BrainHuman Brain

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Baleriola, J., Jean, Y., Troy, C., Hengst, U. Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization. J. Vis. Exp. (100), e52799, doi:10.3791/52799 (2015).

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