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高解像度を使用した脳切片における軸索のローカライズされたmRNAの検出<em>その場での</em>ハイブリダイゼーション
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Neurosciences
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Journal JoVE Neurosciences
Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
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高解像度を使用した脳切片における軸索のローカライズされたmRNAの検出<em>その場での</em>ハイブリダイゼーション

11,568 Views

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11:24 min

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June 17, 2015

DOI:

10.3791/52799-v

DOI:
10.3791/52799-v

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11:24 min
June 17, 2015

•

3 Views
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1College of Physicians and Surgeons,Columbia University

Tags

Axonally Localized MRNAsIn Situ HybridizationRNAscopeAxonal CounterstainFluorescence ImmunohistochemistryHistological DyesAtf4 MRNATranscriptome AnalysisVertebrate AxonsAxon PathfindingAxon BranchingNeurodegenerationSingle-molecule DetectionSubcellular LocalizationMature Mouse BrainHuman Brain

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Baleriola, J., Jean, Y., Troy, C., Hengst, U. Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization. J. Vis. Exp. (100), e52799, doi:10.3791/52799 (2015).

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