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무 세포 생물학 밀봉 Femtoliter 상공 회의소 배열
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Bio-ingénierie
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Journal JoVE Bio-ingénierie
Sealable Femtoliter Chamber Arrays for Cell-free Biology
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무 세포 생물학 밀봉 Femtoliter 상공 회의소 배열

9,298 Views

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13:44 min

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March 11, 2015

DOI:

10.3791/52616-v

DOI:
10.3791/52616-v

•

13:44 min
March 11, 2015

•

3 Views
, , , , , ,
1Bredesen Center,University of Tennessee, Knoxville, 2Center for Nanophase Materials Sciences,Oak Ridge National Laboratory, 3Department of Materials Science and Engineering,University of Tennessee, Knoxville

Summary

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A microfabricated device with sealable femtoliter-volume reaction chambers is described. This report includes a protocol for sealing cell-free protein synthesis reactants inside these chambers for the purpose of understanding the role of crowding and confinement in gene expression.

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Cite this Article

Norred, S. E., Caveney, P. M., Retterer, S. T., Boreyko, J. B., Fowlkes, J. D., Collier, C. P., Simpson, M. L. Sealable Femtoliter Chamber Arrays for Cell-free Biology. J. Vis. Exp. (97), e52616, doi:10.3791/52616 (2015).

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