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Bioengineering

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JoVE Journal Bioengineering
Sealable Femtoliter Chamber Arrays for Cell-free Biology
 

Sealable Femtoliter Chamber Arrays for Cell-free Biology

Article DOI: 10.3791/52616-v • 13:44 min • March 11th, 2015
March 11th, 2015 •
1,2, 1,2, 1,2, 1,2, 2,3, 2, 1,2,3
1Bredesen Center, University of Tennessee, Knoxville, 2Center for Nanophase Materials Sciences, Oak Ridge National Laboratory, 3Department of Materials Science and Engineering, University of Tennessee, Knoxville

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A microfabricated device with sealable femtoliter-volume reaction chambers is described. This report includes a protocol for sealing cell-free protein synthesis reactants inside these chambers for the purpose of understanding the role of crowding and confinement in gene expression.

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Sealable Femtoliter Chamber Arrays Cell-free Biology Cell-free Systems Biological Reactions Macro-scale Bulk Reactors Dynamic Behaviors Efficiencies Living Micro-scale Counterparts Internal Cell Structure Reaction Dynamics Microfabricated Device Poly(dimethylsiloxane) (PDMS) Femtoliter-scale Reaction Chambers Actuated Chambers Cell-Free Protein Synthesis (CFPS) Fluorescent Protein Visualization Of Reaction Kinetics Time-lapse Fluorescent Microscopy Noise Structure Noise Biology Techniques CFPS Gene Circuits
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