Journal
/
Bio-ingénierie
/
Sealable Femtoliter Chamber Arrays for Cell-free Biology
Journal JoVE
Bio-ingénierie
This content is Free Access.
Journal JoVE Bio-ingénierie
Sealable Femtoliter Chamber Arrays for Cell-free Biology

Sealable Femtoliter Chamber Arrays for Cell-free Biology

9,251 Views

•

13:44 min

•

March 11, 2015

DOI:

10.3791/52616-v

DOI:
10.3791/52616-v

•

13:44 min
March 11, 2015

•

3 Views
, , , , , ,
1Bredesen Center,University of Tennessee, Knoxville, 2Center for Nanophase Materials Sciences,Oak Ridge National Laboratory, 3Department of Materials Science and Engineering,University of Tennessee, Knoxville

Summary

Automatically generated

A microfabricated device with sealable femtoliter-volume reaction chambers is described. This report includes a protocol for sealing cell-free protein synthesis reactants inside these chambers for the purpose of understanding the role of crowding and confinement in gene expression.

Explore More Videos

Cell-free BiologyFemtoliter ChambersPDMS DeviceCell-free Protein SynthesisReaction KineticsGene CircuitsNoise Biology

Read Article

Cite this Article

Norred, S. E., Caveney, P. M., Retterer, S. T., Boreyko, J. B., Fowlkes, J. D., Collier, C. P., Simpson, M. L. Sealable Femtoliter Chamber Arrays for Cell-free Biology. J. Vis. Exp. (97), e52616, doi:10.3791/52616 (2015).

Download .ris file

Copy

Share Video

.

Copy