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Dorsal Root Ganglia Neurons and Differentiated Adipose-derived Stem Cells: An In Vitro Co-culture Model to Study Peripheral Nerve Regeneration
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Neurosciences
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Journal JoVE Neurosciences
Dorsal Root Ganglia Neurons and Differentiated Adipose-derived Stem Cells: An In Vitro Co-culture Model to Study Peripheral Nerve Regeneration

Dorsal Root Ganglia Neurons and Differentiated Adipose-derived Stem Cells: An In Vitro Co-culture Model to Study Peripheral Nerve Regeneration

DOI:
10.3791/52543-v

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09:17 min

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February 26, 2015

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, ,
1Centre for Neuroprosthesis,EPFL | STI | IMT/IBI | LSBI, 2Blond McIndoe Research Laboratories, Institute of Inflammation & Repair,The University of Manchester, 3University Hospital of South Manchester

Chapitres

  • 00:05Titre
  • 01:25Adipose-derived Stem Cell (ASC) Harvest
  • 03:32Harvest and Dissociation of Dorsal Root Ganglion (DRG) Neurons
  • 08:00Results: Representative DRG Neuron Images
  • 08:50Conclusion

Summary

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Dorsal root ganglia (DRG) are structures containing the sensory neurons of the peripheral nervous system. When dissociated, they can be co-cultured with SC-like adipose-derived stem cells (ASC), providing a valuable model to study in vitro nerve regeneration and myelination, mimicking the in vivo environment at the injury site.

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Dorsal Root GangliaDRG NeuronsAdipose-derived Stem CellsASCSchwann CellsPeripheral Nerve RegenerationCo-cultureIn Vitro Model

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