Here, we present a protocol to inject ultrasound microbubble contrast agents into living, isolated late-gestation stage murine embryos. This method enables the study of perfusion parameters and of vascular molecular markers within the embryo using contrast-enhanced high-frequency ultrasound imaging.
Denbeigh, J. M., Nixon, B. A., Puri, M. C., Foster, F. S. Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound. J. Vis. Exp. (97), e52520, doi:10.3791/52520 (2015).