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Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound

Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound

14,922 Views

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10:39 min

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March 04, 2015

DOI:

10.3791/52520-v

DOI:
10.3791/52520-v

•

10:39 min
March 04, 2015

•

25 Views
, , ,
1Department of Medical Biophysics,University of Toronto, 2Sunnybrook Research Institute, 3Lunenfeld-Tanenbaum Research Institute,Mount Sinai Hospital, Toronto

Summary

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Here, we present a protocol to inject ultrasound microbubble contrast agents into living, isolated late-gestation stage murine embryos. This method enables the study of perfusion parameters and of vascular molecular markers within the embryo using contrast-enhanced high-frequency ultrasound imaging.

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Denbeigh, J. M., Nixon, B. A., Puri, M. C., Foster, F. S. Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound. J. Vis. Exp. (97), e52520, doi:10.3791/52520 (2015).

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