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Avaliação Compreensiva de Germline Chemical Toxicity Usando o Nemátodo<em> Caenorhabditis elegans</em
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Biologie du développement
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Journal JoVE Biologie du développement
Comprehensive Assessment of Germline Chemical Toxicity Using the Nematode Caenorhabditis elegans

Avaliação Compreensiva de Germline Chemical Toxicity Usando o Nemátodo<em> Caenorhabditis elegans</em

DOI:
10.3791/52445-v

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10:55 min

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February 22, 2015

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, ,
1Institute for Society and Genetics,University of California, Los Angeles, 2Environmental Health Sciences,University of California, Los Angeles, 3California Nanosystems Institute, Department of Molecular and Medical Pharmacology,University of California, Los Angeles

Chapitres

  • 00:05Titre
  • 01:41Establishment of a Synchronized L1 Larvae Populations
  • 03:40Establishment of a Synchronized L4 Larvae Populations
  • 05:03Exposure of Worms to Chemicals in 96-well Plates
  • 07:25Image Acquisition of Gravid Worm in a 384-well Plate
  • 09:23Results: Germline Disruption is Detected by GFP Fluorescence in a C. elegans Reporter Strain Exposed to Nocodazole
  • 10:19Conclusion

Summary

Traduction automatique

Traduction automatique

We describe the detailed steps of a high-throughput chemical assay in the nematode Caenorhabditis elegans used to assess germline toxicity. In this assay, disruption of germline function following chemical exposure is monitored using a fluorescent reporter specific to aneuploid embryos.

Tags

Germline ToxicityCaenorhabditis ElegansNematode ModelChromosome SegregationAneuploid EmbryosGFP ReporterHigh-throughput ScreeningReproductive ToxicityEnvironmental Health

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