Journal
/
Biologie du développement
/
Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells
Journal JoVE
Biologie du développement
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie du développement
Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells

DOI:
10.3791/52298-v

•

12:06 min

•

January 28, 2015

•

, , , , ,
1Translational Neuroscience Center, National Institute of Neurological Disorders and Stroke,National Institutes of Health, 2Laboratory of Molecular Medicine and Neuroscience, National Institute of Neurological Disorders and Stroke,National Institutes of Health

Chapitres

  • 00:05Titre
  • 01:44Culture of CD34 Cells
  • 03:14Sendai Virus Transfection
  • 05:24Neural Stem Cell Induction
  • 07:20Freezing Neural Stem Cells
  • 08:43Neural Cell Differentiation
  • 10:08Results: Expressed Markers in Induced and Differentiated Neural Stem Cells
  • 11:05Conclusion

Summary

Traduction automatique

Traduction automatique

A method was developed to directly derive human neural stem cells from hematopoietic progenitor cells enriched from peripheral blood cells.

Tags

Human Neural Stem CellsPeripheral BloodHematopoietic Progenitor CellsInduced Pluripotent Stem CellsSendai VirusNeural Progenitor MarkersNeural DifferentiationNeurological Disorders

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Derivation of Cardiac Progenitor Cells from Embryonic Stem Cells

Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord

Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells

Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood

Single-cell Photoconversion in Living Intact Zebrafish

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

Read Article