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Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells
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发育生物学
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JoVE 杂志 发育生物学
Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells

DOI:
10.3791/52298-v

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12:06 min

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January 28, 2015

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, , , , ,
1Translational Neuroscience Center, National Institute of Neurological Disorders and Stroke,National Institutes of Health, 2Laboratory of Molecular Medicine and Neuroscience, National Institute of Neurological Disorders and Stroke,National Institutes of Health

Chapters

  • 00:05Title
  • 01:44Culture of CD34 Cells
  • 03:14Sendai Virus Transfection
  • 05:24Neural Stem Cell Induction
  • 07:20Freezing Neural Stem Cells
  • 08:43Neural Cell Differentiation
  • 10:08Results: Expressed Markers in Induced and Differentiated Neural Stem Cells
  • 11:05Conclusion

Summary

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A method was developed to directly derive human neural stem cells from hematopoietic progenitor cells enriched from peripheral blood cells.

Tags

Human Neural Stem CellsPeripheral BloodHematopoietic Progenitor CellsInduced Pluripotent Stem CellsSendai VirusNeural Progenitor MarkersNeural DifferentiationNeurological Disorders

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