Bir Geliştirilmesi<em> In vitro</emEtkileşimini incelemek için> bir model sistem<em> Equus</em><em> Caballus</emYüksek afiniteli reseptör FcεRI> ile IgE
Journal JoVE
Immunologie et infection Author Produced
Bir Geliştirilmesi<em> In vitro</emEtkileşimini incelemek için> bir model sistem<em> Equus</em><em> Caballus</emYüksek afiniteli reseptör FcεRI> ile IgE
The current study describes the development and applications of a genetically engineered assay system based on the transfection of rat basophilic leukemia cells with the equine FcεRIα gene. Transfected cells express a functional receptor where the release of mediators of the allergic response can be activated by IgE and antigen.
Sabban, S., Ye, H., Helm, B. Development of an in vitro model system for studying the interaction of Equuscaballus IgE with its high-affinity receptor FcεRI. J. Vis. Exp. (93), e52222, doi:10.3791/52222 (2014).