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Methods for Cell-attached Capacitance Measurements in Mouse Adrenal Chromaffin Cell
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Journal JoVE Neurosciences
Methods for Cell-attached Capacitance Measurements in Mouse Adrenal Chromaffin Cell

Methods for Cell-attached Capacitance Measurements in Mouse Adrenal Chromaffin Cell

DOI:
10.3791/52024-v

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08:24 min

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October 22, 2014

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, ,
1Department of Biological Sciences,University of Illinois at Chicago

Chapitres

  • 00:05Titre
  • 01:07Adrenal Gland Isolation
  • 02:52Tissue Digestion
  • 03:50Cell Dissociation and Plating
  • 05:16Cell Identification and Gigaohm Seal Formation
  • 06:41Cell-attached Capacitance Recordings
  • 07:34Results: Cell-attached Capacitance Recording Shows Endocytosis in Chromaffin Cells
  • 07:52Conclusion

Summary

Traduction automatique

Traduction automatique

After exocytosis, fused plasma membrane is retrieved through a process known as endocytosis. This mechanism reforms new synaptic vesicles for the next round of release. Individual endocytic events are captured and analyzed through the use of the cell-attached capacitance recordings in mouse adrenal chromaffin cells.

Tags

Keywords: Cell-attached CapacitanceMouse Adrenal Chromaffin CellNeuronal TransmissionExocytosisEndocytosisSynaptic VesiclesSingle Vesicle EventsElectrophysiology

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