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Methods for Cell-attached Capacitance Measurements in Mouse Adrenal Chromaffin Cell
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Kapitel
- 00:05Titel
- 01:07Adrenal Gland Isolation
- 02:52Tissue Digestion
- 03:50Cell Dissociation and Plating
- 05:16Cell Identification and Gigaohm Seal Formation
- 06:41Cell-attached Capacitance Recordings
- 07:34Results: Cell-attached Capacitance Recording Shows Endocytosis in Chromaffin Cells
- 07:52Conclusion
After exocytosis, fused plasma membrane is retrieved through a process known as endocytosis. This mechanism reforms new synaptic vesicles for the next round of release. Individual endocytic events are captured and analyzed through the use of the cell-attached capacitance recordings in mouse adrenal chromaffin cells.
