Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Paired Whole Cell Recordings in Organotypic…
JoVE Journal
Neuroscience

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Neuroscience
Paired Whole Cell Recordings in Organotypic Hippocampal Slices
 

Paired Whole Cell Recordings in Organotypic Hippocampal Slices

Article DOI: 10.3791/51958-v • 09:23 min • September 28th, 2014
September 28th, 2014 •
1, 2, *2, *1
1Department of Physiology and Centre for Brain Research, University of Auckland, 2Department of Molecular and Cellular Physiology, Stanford University
* These authors contributed equally

Chapitres

résumé
Automatic Translation

Pair recordings are simultaneous whole cell patch clamp recordings from two synaptically connected neurons, enabling precise electrophysiological and pharmacological characterization of the synapses between individual neurons. Here we describe the detailed methodology and requirements for establishing this technique in organotypic hippocampal slice cultures in any laboratory equipped for electrophysiology.

Tags

Keywords: Paired Whole Cell Recordings Organotypic Hippocampal Slices Synaptic Connections Electrophysiology Patch Clamp CA3-CA3 Pyramidal Cell Pairs CA3-CA1 Pairs Synaptic Transmission Synaptic Plasticity
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter