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在小鼠B淋巴细胞染色体畸变所PNA-FISH快速分析
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Immunologie et infection
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Journal JoVE Immunologie et infection
Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH
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在小鼠B淋巴细胞染色体畸变所PNA-FISH快速分析

16,744 Views

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07:54 min

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August 19, 2014

DOI:

10.3791/51806-v

DOI:
10.3791/51806-v

•

07:54 min
August 19, 2014

•

3 Views
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1Department of Molecular Biology and Biochemistry,Rutgers, the State University of New Jersey

Summary

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DNA修复途径对于维持基因组的完整性，防止基因突变和癌症至关重要。该协议的目的是通过直接观察染色体畸变的量化基因组不稳定性在中期从原位杂交（FISH）对端粒DNA重复使用荧光小鼠B细胞的扩散。

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Chromosome AberrationsMouse B LymphocytesPNA-FISHDNA RepairGenomic InstabilityTumorigenesisMetaphase Chromosome SpreadsPloidyMitotic IndexPrimary Cell PopulationTransformed FibroblastsPatient Cell Lines

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Cite this Article

Misenko, S. M., Bunting, S. F. Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH. J. Vis. Exp. (90), e51806, doi:10.3791/51806 (2014).

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