Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Isolement de cellules murines ganglions lymphatiques stromales
30,621 Views
•
05:47 min
•
August 19, 2014
DOI:
Summary
Automatically generated
Isolation of lymph node stromal cells is a multistep procedure including enzymatic digestion and mechanical disaggregation to obtain fibroblastic reticular cells, lymphatic and blood endothelial cells. In the described procedure, a short digestion is combined with automated mechanical disaggregation to minimize surface marker degradation of viable lymph node stromal cells.
Read Article
Cite this Article
Broggi, M. A. S., Schmaler, M., Lagarde, N., Rossi, S. W. Isolation of Murine Lymph Node Stromal Cells. J. Vis. Exp. (90), e51803, doi:10.3791/51803 (2014).
Copy