Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 01:49In Situ Staining of Cerebral Sections with MHC Class II (IAs)/PLP 139-151 Dextramers
- 06:28Analysis of Dextramer+ (dext+) CD4+ T Cells by Laser Scanning Confocal Microscopy (LSCM)
- 10:50Results: In Situ Detection of Antigen-specific Autoreactive CD4 T Cells by Direct Staining with MHC Class II Dextramers
- 12:29Conclusion
Tags
MHC Class II DextramersAutoreactive CD4 T CellsExperimental Autoimmune Encephalomyelitis (EAE)Experimental Autoimmune Myocarditis (EAM)Laser Scanning Confocal Microscope (LSCM)Myelin Proteolipid Protein (PLP) 139-151Cardiac Myosin Heavy Chain-α (Myhc) 334-352Theiler’s Murine Encephalomyelitis Virus (TMEV) 70-86Bovine Ribonuclease (RNase) 43-56In Situ DetectionQuantitative Analysis
