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In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers
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In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers

In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers

11,504 Views

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13:10 min

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August 01, 2014

DOI:

10.3791/51679-v

DOI:
10.3791/51679-v

•

13:10 min
August 01, 2014

•

4 Views
, , , , , ,
1School of Veterinary Medicine and Biomedical Sciences,University of Nebraska, Lincoln, 2Center for Biotechnology,University of Nebraska, Lincoln, 3Nebraska Center for Virology and School of Biological Sciences,University of Nebraska, Lincoln

Summary

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The protocol to detect self-reactive CD4 T cells in brain and heart by direct staining with major histocompatibility complex class II dextramers has been described in this report. For comprehensive analysis, a reliable method to enumerate the frequencies of antigen-specific CD4+ T cells in situ is also devised.

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Cite this Article

Massilamany, C., Gangaplara, A., Jia, T., Elowsky, C., Li, Q., Zhou, Y., Reddy, J. In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers. J. Vis. Exp. (90), e51679, doi:10.3791/51679 (2014).

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