/
/
Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons
12,157 Views
•
09:36 min
•
May 12, 2014
DOI:
1Department of Physiological Genomics, Institute of Physiology,Ludwig Maximilians University Munich, 2Tumor Biology Lab, Neurosurgical Clinic,Ludwig-Maximilians University Munich, 3Institut für Biochemie, Emil-Fischer-Zentrum,Friedrich-Alexander-Universität Erlangen-Nürnberg, 4Institute of Physiological Chemistry and Focus Program Translational Neuroscience,Johannes Gutenberg University Mainz
Summary
Automatically generated
Targeting brain-resident cells for direct lineage-reprogramming offers new perspectives for brain repair. Here we describe a protocol of how to prepare cultures enriched for brain-resident pericytes from the adult human cerebral cortex and convert these into induced neurons by retrovirus-mediated expression of the transcription factors Sox2 and Ascl1.
Read Article
Cite this Article
Karow, M., Schichor, C., Beckervordersandforth, R., Berninger, B. Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons. J. Vis. Exp. (87), e51433, doi:10.3791/51433 (2014).
Copy