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全球合成的RNA在单细胞水平以下的低氧分析
Journal JoVE
Biologie
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Journal JoVE Biologie
Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia
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全球合成的RNA在单细胞水平以下的低氧分析

12,078 Views

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14:53 min

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May 13, 2014

DOI:

10.3791/51420-v

DOI:
10.3791/51420-v

•

14:53 min
May 13, 2014

•

5 Views
, , , , ,
1Centre for Gene Regulation and Expression, College of Life Sciences,University of Dundee, UK

Summary

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我们描述了全球性RNA合成缺氧使用成像分析技术。以前没有缺氧条件下进行的RNA点击化学标记，并允许全球的RNA变化的可视化在单细胞水平。这种方法将与现有的平均值RNA技术，允许的细胞 - 细胞间的变化在全球RNA合成的直接可视化。

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Cite this Article

Biddlestone, J., Druker, J., Shmakova, A., Ferguson, G., Swedlow, J. R., Rocha, S. Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia. J. Vis. Exp. (87), e51420, doi:10.3791/51420 (2014).

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