Journal
/
Immunologie et infection
/
A Protocol for Analyzing Hepatitis C Virus Replication
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
A Protocol for Analyzing Hepatitis C Virus Replication

A Protocol for Analyzing Hepatitis C Virus Replication

23,843 Views

•

13:04 min

•

June 26, 2014

DOI:

10.3791/51362-v

DOI:
10.3791/51362-v

•

13:04 min
June 26, 2014

•

9 Views
, ,
1Liver Program at Regenerative Medicine Institute, Department of Biomedical Sciences, Department of Surgery,Cedars-Sinai Medical Center, 2Department of Surgery,David Geffen School of Medicine at UCLA

Summary

Automatically generated

Hepatitis C Virus (HCV) is a major human pathogen that causes liver disorders, including cirrhosis and cancer. An HCV infectious cell culture system is essential for understanding the molecular mechanism of HCV replication and developing new therapeutic approaches. Here we describe a protocol to investigate various stages of the HCV replication cycle.

Explore More Videos

Hepatitis C VirusHCVFlaviviridaeViral ReplicationCell Culture SystemHuh-7 CellsQuantitative RT-PCRDouble-stranded RNAWestern BlotImmunofluorescenceLuciferase AssayViral EntryGenome ReplicationPackagingEgress

Read Article

Cite this Article

Ren, S., Contreras, D., Arumugaswami, V. A Protocol for Analyzing Hepatitis C Virus Replication. J. Vis. Exp. (88), e51362, doi:10.3791/51362 (2014).

Download .ris file

Copy

Share Video

.

Copy