Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
A Protocol for Analyzing Hepatitis C Virus Replication
Capítulos
- 00:05Título
- 02:06In Vitro HCV RNA Transcription
- 03:24HCV RNA Transfection and Sample Collection
- 05:42RT-qPCR for Assessing HCV Genome Copies and Western Blot Analysis for Detecting HCV Protein Expression
- 07:31Immunofluorescence Assay
- 08:52Measuring Virus Titer
- 09:56Results: Analysis of Hepatitis C Virus Replication
- 11:50Conclusion
Hepatitis C Virus (HCV) is a major human pathogen that causes liver disorders, including cirrhosis and cancer. An HCV infectious cell culture system is essential for understanding the molecular mechanism of HCV replication and developing new therapeutic approaches. Here we describe a protocol to investigate various stages of the HCV replication cycle.
