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Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
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Immunologie et infection
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Journal JoVE Immunologie et infection
Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
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Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking

6,050 Views

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11:33 min

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December 17, 2013

DOI:

10.3791/51217-v

DOI:
10.3791/51217-v

•

11:33 min
December 17, 2013

•

2 Views
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1Genetics and Biochemistry Branch of the National Institute of Diabetes and Digestive and Kidney Diseases,National Institutes of Health

Tags

Site-specific CrosslinkingAmber SuppressionP-benzoylphenylalaninePhotocrosslinkingProtein-protein InteractionsBacterial Virulence FactorAssemblyPulse-chase LabelingImmunoprecipitationMass Spectrometry

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Pavlova, O., Ieva, R., Bernstein, H. D. Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking. J. Vis. Exp. (82), e51217, doi:10.3791/51217 (2013).

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