Journal
/
Médecine
/
Cell-based Assay Protocol for the Prognostic Prediction of Idiopathic Scoliosis Using Cellular Dielectric Spectroscopy
Journal JoVE
Médecine
This content is Free Access.
Journal JoVE Médecine
Cell-based Assay Protocol for the Prognostic Prediction of Idiopathic Scoliosis Using Cellular Dielectric Spectroscopy

Cell-based Assay Protocol for the Prognostic Prediction of Idiopathic Scoliosis Using Cellular Dielectric Spectroscopy

DOI:
10.3791/50768-v

•

08:08 min

•

October 16, 2013

•

, ,
1Viscogliosi Laboratory in Molecular Genetics of Musculoskeletal Diseases,Sainte-Justine University Hospital Research Center, 2Department of Stomatology, Faculty of Dentistry and Department of Biochemistry, Faculty of Medicine,Université de Montréal

Chapitres

  • 00:05Titre
  • 02:04PBMC Culture
  • 03:38Treat Cells with Recombinant OPN
  • 05:49Results: Prognosis of Idiopathic Scoliosis
  • 07:44Conclusion

Summary

Traduction automatique

Traduction automatique

A structured protocol is presented for a cell-based assay as a functional test to predict the prognosis of idiopathic scoliosis using cellular dielectric spectroscopy (CDS). The assay can be performed with fresh or frozen peripheral blood mononuclear cells (PBMCs) and the procedure is completed within 4 days.

Tags

Cell-based AssayPrognostic PredictionIdiopathic ScoliosisCellular Dielectric SpectroscopyPeripheral Blood Mononuclear CellsGi ProteinsGs ProteinsOsteopontinPhytohemmaglutininSomatostatinIsoproterenol

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

An Experimental Paradigm for the Prediction of Post-Operative Pain (PPOP)

A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation

The Trier Social Stress Test Protocol for Inducing Psychological Stress

High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum

Focus Formation: A Cell-based Assay to Determine the Oncogenic Potential of a Gene

Diffuse Optical Spectroscopy for the Quantitative Assessment of Acute Ionizing Radiation Induced Skin Toxicity Using a Mouse Model

Phosphorus-31 Magnetic Resonance Spectroscopy: A Tool for Measuring In Vivo Mitochondrial Oxidative Phosphorylation Capacity in Human Skeletal Muscle

Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System

Quantification of Optic Nerve Cross Sectional Area on MRI: A Novel Protocol using Fiji Software

A Real-World High-Intensity Interval Training Protocol for Cardiorespiratory Fitness Improvement

Read Article