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Cell-based Assay Protocol for the Prognostic Prediction of Idiopathic Scoliosis Using Cellular Dielectric Spectroscopy
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Médecine
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Journal JoVE Médecine
Cell-based Assay Protocol for the Prognostic Prediction of Idiopathic Scoliosis Using Cellular Dielectric Spectroscopy

Cell-based Assay Protocol for the Prognostic Prediction of Idiopathic Scoliosis Using Cellular Dielectric Spectroscopy

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08:08 min

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October 16, 2013

DOI:

10.3791/50768-v

DOI:
10.3791/50768-v

•

08:08 min
October 16, 2013

•

1 Views
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1Viscogliosi Laboratory in Molecular Genetics of Musculoskeletal Diseases,Sainte-Justine University Hospital Research Center, 2Department of Stomatology, Faculty of Dentistry and Department of Biochemistry, Faculty of Medicine,Université de Montréal

Summary

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A structured protocol is presented for a cell-based assay as a functional test to predict the prognosis of idiopathic scoliosis using cellular dielectric spectroscopy (CDS). The assay can be performed with fresh or frozen peripheral blood mononuclear cells (PBMCs) and the procedure is completed within 4 days.

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Akoume, M., Franco, A., Moreau, A. Cell-based Assay Protocol for the Prognostic Prediction of Idiopathic Scoliosis Using Cellular Dielectric Spectroscopy. J. Vis. Exp. (80), e50768, doi:10.3791/50768 (2013).

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