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Cell-based Assay Protocol for the Prognostic Prediction of Idiopathic Scoliosis Using Cellular Dielectric Spectroscopy
JoVE Revista
Medicina
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JoVE Revista Medicina
Cell-based Assay Protocol for the Prognostic Prediction of Idiopathic Scoliosis Using Cellular Dielectric Spectroscopy

Cell-based Assay Protocol for the Prognostic Prediction of Idiopathic Scoliosis Using Cellular Dielectric Spectroscopy

DOI:
10.3791/50768-v

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08:08 min

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October 16, 2013

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, ,
1Viscogliosi Laboratory in Molecular Genetics of Musculoskeletal Diseases,Sainte-Justine University Hospital Research Center, 2Department of Stomatology, Faculty of Dentistry and Department of Biochemistry, Faculty of Medicine,Université de Montréal

Capítulos

  • 00:05Título
  • 02:04PBMC Culture
  • 03:38Treat Cells with Recombinant OPN
  • 05:49Results: Prognosis of Idiopathic Scoliosis
  • 07:44Conclusion

Summary

Traducción Automática

Traducción Automática

A structured protocol is presented for a cell-based assay as a functional test to predict the prognosis of idiopathic scoliosis using cellular dielectric spectroscopy (CDS). The assay can be performed with fresh or frozen peripheral blood mononuclear cells (PBMCs) and the procedure is completed within 4 days.

Tags

Cell-based AssayPrognostic PredictionIdiopathic ScoliosisCellular Dielectric SpectroscopyPeripheral Blood Mononuclear CellsGi ProteinsGs ProteinsOsteopontinPhytohemmaglutininSomatostatinIsoproterenol

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