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Compact Quantum Dots for Single-molecule Imaging
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October 09, 2012
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We describe the preparation of colloidal quantum dots with minimized hydrodynamic size for single-molecule fluorescence imaging. Compared to conventional quantum dots, these nanoparticles are similar in size to globular proteins and are optimized for single-molecule brightness, stability against photodegradation, and resistance to nonspecific binding to proteins and cells.
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Compact Quantum DotsSingle-molecule ImagingBiomolecular FunctionSpatial HeterogeneityTemporal HeterogeneityEpifluorescence MicroscopyTotal Internal Reflection Fluorescence MicroscopyFluorescent TagDyeProteinBeadQuantum DotHigh Photon FluxesFluorescence ImagingSignal StabilityHydrodynamic SizeDiffusion BehaviorSemiconductor MaterialsPhotodegradation
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Smith, A. M., Nie, S. Compact Quantum Dots for Single-molecule Imaging. J. Vis. Exp. (68), e4236, doi:10.3791/4236 (2012).
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