Compact Quantum Dots for Single-molecule Imaging

Andrew M. Smith; Shuming Nie

doi:10.3791/4236

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工程学

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Compact Quantum Dots for Single-molecule Imaging

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工程学

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JoVE 杂志工程学

Compact Quantum Dots for Single-molecule Imaging

17,869 Views

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17:14 min

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October 09, 2012

DOI:

10.3791/4236-v

DOI:

10.3791/4236-v

•

17:14 min

October 09, 2012

•

5 Views

Andrew M. Smith¹, Shuming Nie^1,2

¹Department of Biomedical Engineering,Emory University, ²Department of Chemistry,Georgia Institute of Technology

Summary

We describe the preparation of colloidal quantum dots with minimized hydrodynamic size for single-molecule fluorescence imaging. Compared to conventional quantum dots, these nanoparticles are similar in size to globular proteins and are optimized for single-molecule brightness, stability against photodegradation, and resistance to nonspecific binding to proteins and cells.

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Compact Quantum Dots Single-molecule Imaging Biomolecular Function Spatial Heterogeneity Temporal Heterogeneity Epifluorescence Microscopy Total Internal Reflection Fluorescence Microscopy Fluorescent Tag Dye Protein Bead Quantum Dot High Photon Fluxes Fluorescence Imaging Signal Stability Hydrodynamic Size Diffusion Behavior Semiconductor Materials Photodegradation

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Compact Quantum Dots for Single-molecule Imaging

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