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Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors
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Immunologie et infection
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Journal JoVE Immunologie et infection
Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors

11,442 Views

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16:49 min

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July 16, 2012

DOI:

10.3791/4029-v

DOI:
10.3791/4029-v

•

16:49 min
July 16, 2012

•

2 Views
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1Division of Experimental Virology,Twincore, Centre for Experimental and Clinical Infection Research, 2Aaron Diamond AIDS Research Center, Laboratory of Retrovirology,The Rockefeller University, NY

Tags

Heterokaryon FormationHCV Restriction FactorsHCV ReplicationHost-rangeChimpanzeesHCV RNA ReplicationNon-hepatic Cell LinesMurine Cell LinesSelection ProceduresHCV Replicon ConstructsAntibiotic-selectable MarkersIn Vitro ResearchHepatoma Cell LinesViral Life CycleSpecies TropismImmunocompetent Small Animal ModelGenetic IncompatibilityHost Dependency FactorsRestriction FactorsConditional Trans-complementation MethodsSomatic Cell FusionViral Replication CycleHeterokaryonsTrans-complementationInfectious Viral ProgenySubgenomic HCV Rep

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Frentzen, A., Hueging, K., Bitzegeio, J., Pietschmann, T., Steinmann, E. Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors. J. Vis. Exp. (65), e4029, doi:10.3791/4029 (2012).

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