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Local and Global Methods of Assessing Thermal Nociception in Drosophila Larvae
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1Department of Biochemistry and Molecular Biology,The University of Texas MD Anderson Cancer Center, 2Scholars Academy/MARC Scholar,University of Houston-Downtown, 3Genes and Development Graduate Program,University of Texas Graduate School of Biomedical Sciences, 4Neuroscience Graduate Program,University of Texas Graduate School of Biomedical Sciences
Chapitres
- 00:05Titre
- 02:02Preparation of Larvae
- 02:37Etherization and UV Irradiation
- 04:11Local Heat Probe Assay
- 05:23Global Heat Plate Assay
- 07:49Results: Quantified Behavioral Responses in Local and Global Thermal Nociception Assays
- 10:15Conclusion
In this article, we demonstrate assays to study thermal nociception in Drosophila larvae. One assay involves spatially-restricted (local) stimulation of thermal nociceptors1,2 while the second involves a wholesale (global) activation of most or all such neurons3. Together, these techniques allow visualization and quantification of the behavioral functions of Drosophila nociceptive sensory neurons.
