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Local and Global Methods of Assessing Thermal Nociception in Drosophila Larvae
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Local and Global Methods of Assessing Thermal Nociception in Drosophila Larvae
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May 18, 2012
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1Department of Biochemistry and Molecular Biology,The University of Texas MD Anderson Cancer Center, 2Scholars Academy/MARC Scholar,University of Houston-Downtown, 3Genes and Development Graduate Program,University of Texas Graduate School of Biomedical Sciences, 4Neuroscience Graduate Program,University of Texas Graduate School of Biomedical Sciences
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In this article, we demonstrate assays to study thermal nociception in Drosophila larvae. One assay involves spatially-restricted (local) stimulation of thermal nociceptors1,2 while the second involves a wholesale (global) activation of most or all such neurons3. Together, these techniques allow visualization and quantification of the behavioral functions of Drosophila nociceptive sensory neurons.
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Chattopadhyay, A., Gilstrap, A. V., Galko, M. J. Local and Global Methods of Assessing Thermal Nociception in Drosophila Larvae. J. Vis. Exp. (63), e3837, doi:10.3791/3837 (2012).
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