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Immunologie et infection
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Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model
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Immunologie et infection
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Journal JoVE Immunologie et infection
Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model

Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model

DOI:
10.3791/3697-v

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10:29 min

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August 29, 2012

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,
1Department of Surgery,The Ohio State University Medical Center

Chapitres

  • 00:05Titre
  • 01:10Lethal Irradiation and Splenocyte Preparation
  • 03:12Bone Marrow Preparation and Removal
  • 05:16CD3 Depletion
  • 06:08L2G85.B6 (LUX+) CD8+ T Cell Purification and Injection Preparation
  • 07:48Bioluminescent Imaging
  • 08:12Résultats
  • 10:12Conclusion

Summary

Traduction automatique

Traduction automatique

Murine bone marrow transplantation is a widely used technique to study immunological mechanisms governing graft-versus-host disease in humans. The ability to monitor T cell trafficking patterns in vivo allows for detailed analysis of the development and perpetuation of T cell responses during graft-versus-host disease.

Tags

Graft-versus-host DiseaseBone Marrow TransplantHematological DeficienciesAlloreactive T CellsBioluminescent ImagingT Cell TraffickingL2G85.B6 Transgenic MiceLuciferaseSpecific T Cell SubsetsGVHD PromotionTime PointsXenogen IVIS CCD CameraLiving Image Software

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