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Isolering og Udvidelse af menneskelige glioblastom multiforme tumorceller Brug af Neurosphere Assay
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Neurosciences
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Journal JoVE Neurosciences
Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay

Isolering og Udvidelse af menneskelige glioblastom multiforme tumorceller Brug af Neurosphere Assay

29,284 Views

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09:24 min

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October 30, 2011

DOI:

10.3791/3633-v

DOI:
10.3791/3633-v

•

09:24 min
October 30, 2011

•

14 Views
, , , , ,
1Department of Neurosurgery,University of Florida , 2Department of Anatomical Sciences,Shiraz University of Medical Sciences

Tags

IsolationExpansionHuman Glioblastoma MultiformeTumor CellsNeurosphere AssayStem-like CellsBreastLungColonProstateBrainTumor Initiating Cell Population(s)Tumor FormationProgressionRecurrenceTherapeutic ApproachesNeurosphere Culture MethodChemical DigestionMechanical DissociationSingle Cell SuspensionNSA CulturePrimary Neurospheres

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Azari, H., Millette, S., Ansari, S., Rahman, M., Deleyrolle, L. P., Reynolds, B. A. Isolation and Expansion of Human Glioblastoma Multiforme Tumor Cells Using the Neurosphere Assay. J. Vis. Exp. (56), e3633, doi:10.3791/3633 (2011).

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