Journal
/
Neurosciences
/
كشف عن نقص الأكسجة Microregional في اللحاء المخي الفأر بواسطة التصوير ثنائي الفوتون من الإسفار NADH الذاتية
Journal JoVE
Neurosciences
This content is Free Access.
Journal JoVE Neurosciences
Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence
Please note that all translations are automatically generated. Click here for the English version.

كشف عن نقص الأكسجة Microregional في اللحاء المخي الفأر بواسطة التصوير ثنائي الفوتون من الإسفار NADH الذاتية

15,771 Views

•

12:03 min

•

February 21, 2012

DOI:

10.3791/3466-v

DOI:
10.3791/3466-v

•

12:03 min
February 21, 2012

•

7 Views
, , , , ,
1Department of Microbiology and Immunology,University of Rochester Medical Center, 2Center for Neural Development and Disease,University of Rochester Medical Center, 3Deptartment of Neurology, Center for Neural Development and Disease,University of Rochester Medical Center

Tags

DetectionMicroregional HypoxiaMouse Cerebral CortexTwo-photon ImagingEndogenous NADH FluorescenceOxygen SupplyBlood FlowVisualized Experimental ProtocolMethodological ProtocolPerivascular Oxygen GradientsNon-linear RelationshipNicotinamide Adenine Dinucleotide (NADH)Fluorescence IntensityOxygen Partial PressureTwo-photon ExcitationTexas-Red DextranAdvantagesStandard Two-photon In Vivo Imaging EquipmentContinuous MonitoringDepth ResolutionVulnerable Watershed AreasDecline In Vascular Oxygen SupplyMicroregional Cortical Oxygenation

Read Article

Cite this Article

Polesskaya, O., Sun, A., Salahura, G., Silva, J. N., Dewhurst, S., Kasischke, K. Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence. J. Vis. Exp. (60), e3466, doi:10.3791/3466 (2012).

Download .ris file

Copy

Share Video

.

Copy