/

Immunologie et infection

/

Epstein - Barr病毒生长转化的淋巴细胞系的建立

Journal JoVE
Immunologie et infection

This content is Free Access.

Journal JoVE Immunologie et infection

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Please note that all translations are automatically generated. Click here for the English version.

Epstein - Barr病毒生长转化的淋巴细胞系的建立

DOI:

•

06:38 min

•

November 08, 2011

•

Joyce Hui-Yuen², Shane McAllister², Siva Koganti, Erik Hill, Sumita Bhaduri-McIntosh^2,3,4

¹Stony Brook Children’s Hospital,State University of New York at Stony Brook, ²Department of Pediatrics,State University of New York at Stony Brook, ³Department of Molecular Genetics,State University of New York at Stony Brook, ⁴Department of Microbiology,State University of New York at Stony Brook

Chapitres

01:35Isolation of Peripheral Blood Mononuclear Cells (PBMC)
02:53Infection with Epstein-Barr Virus (EBV)
03:47Expansion and Cryopreservation of Lymphoblastoid Cell Lines (LCL)
04:46Success of Outcome can be Predicted by Flow Cytometric Analysis of Cells
05:58Conclusion

Summary

Traduction automatique

我们描述了一种用于发电使用EB病毒转化的B细胞株的方法。我们也说明了一种新的分析，可以识别注定要进行改造，早在感染后3天的B细胞。

Tags

Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines EBV Infection B Cell Proliferation Immortalization EBV Latency Virus-driven B Cell Proliferation Tumorigenesis Immunologic Assays Human Monoclonal Antibodies Mitogens Phytohemagglutinin Lipopolysaccharide Pokeweed Mitogen Cyclosporin A T Cell-mediated Killing High Titer EBV Immunosuppressive Agent Peripheral Blood Mononuclear Cells

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

胸腺淋巴瘤的T细胞系的推导 ATM^{- / -</sup} P53^{- / -</sup}鼠标

扩大目标，脐血细胞毒性T淋巴细胞，巨细胞病毒，EB病毒，腺病毒

顺行运输Alphaherpes病毒传播的活细胞成像

中性粒细胞分离和分析，以确定他们的淋巴瘤细胞的敏感性对治疗药物的作用

RNA的疗效和毒性的评价基因或药物治疗靶向HIV-1的生产使用

一个简单的红细胞溶解法对B淋巴母细胞系的建立

慢性活性泼斯坦-巴尔病毒感染患者 NK 和 T 细胞系建立的高效简便方法

猪全血表达编码和非编码 rna 类的鉴定

果蝇病毒感染的建立及宿主病毒相互作用分析

体外ELISA测试评估狂犬病疫苗效力

Read Article