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ナイフエッジスキャン顕微鏡用試料作製、イメージング、および解析プロトコル
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10:25 min
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December 09, 2011
DOI:
1Department of Computer Science and Engineering,Texas A&M University, 2Beckman Institute for Advanced Science and Technology,University of Illinois, 3Department of Electrical and Computer Engineering,Kettering University, 43Scan, 5Department of Veterinary Integrative Biosciences,Texas A&M University
Summary
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脳標本の準備からデータの可視化と分析に、ナイフエッジの走査型顕微鏡を使用してシリアルのセクショニング画像への完全なプロセスが記載されている。この手法は、現在のマウスの脳のデータを取得するために使用されますが、それは他の臓器、他の種に適用可能です。
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Cite this Article
Choe, Y., Mayerich, D., Kwon, J., Miller, D. E., Sung, C., Chung, J. R., Huffman, T., Keyser, J., Abbott, L. C. Specimen Preparation, Imaging, and Analysis Protocols for Knife-edge Scanning Microscopy. J. Vis. Exp. (58), e3248, doi:10.3791/3248 (2011).
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