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Chapitres
- 00:05Titre
- 01:02Stable Expression of CMV-Driven Renilla Luciferase
- 01:36Expression, Selection, and Functional Verification of Inducible Promoter-Driven Firefly Luciferase
- 02:32Establishing 4T1 Primary Mammary Tumors
- 03:25Initial Dual Luminescent Imaging
- 04:22Longitudinal Luminescent Imaging
- 05:17Dual Bioluminescent Imaging of Two Unique Cell Types in a Single Animal
- 06:16In vivo RLRs from Dual Bioluminescent Imaging
- 07:10Conclusion
Hier beschreiben wir die Methoden zu konstruieren, zu visualisieren und zu quantifizieren Biolumineszenzreaktionen sowohl Glühwürmchen und Renilla Luciferase Enzymen bei metastasierendem Brustkrebs-Zellen während deren Wachstum und Metastasierung ausgedrückt<em> In vivo</em>.
Tags
In VivoDual Substrate Bioluminescent ImagingBreast Cancer CellsPrimary TumorsMetastatic SitesIn Vivo Bioluminescent Imaging TechnologiesTumor GrowthLongitudinal StudyResearchersBreast Cancer MetastasisCell Signaling SystemsGene ExpressionTumor DevelopmentMetastatic ProgressionLuciferase EnzymesFirefly LuciferaseSea Pansy Luciferase
