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Autologous Endothelial Progenitor Cell-Seeding Technology and Biocompatibility Testing For Cardiovascular Devices in Large Animal Model
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Autologous Endothelial Progenitor Cell-Seeding Technology and Biocompatibility Testing For Cardiovascular Devices in Large Animal Model
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September 09, 2011
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A method for seeding titanium blood-contacting biomaterials with autologous cells and testing biocompatibility is described. This method uses endothelial progenitor cells and titanium tubes, seeded within minutes of surgical implantation into porcine venae cavae. This technique is adaptable to many other implantable biomedical devices.
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Autologous Endothelial Progenitor Cell-seeding TechnologyBiocompatibility TestingCardiovascular DevicesLarge Animal ModelImplantable DevicesArtificial MaterialsThromboemboli FormationTi TubesBlood-derived Human Or Porcine Endothelial Progenitor Cells (EPCs)Inferior Vena Cava (IVC)BiocompatibilityProthrombotic EnvironmentBare Metal SurfacesEndothelialize DevicesAntithrombotic FunctionPeripheral BloodMononuclear Cell FractionCultureLaparotomy
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Cite this Article
Jantzen, A. E., Lane, W. O., Gage, S. M., Haseltine, J. M., Galinat, L. J., Jamiolkowski, R. M., Lin, F., Truskey, G. A., Achneck, H. E. Autologous Endothelial Progenitor Cell-Seeding Technology and Biocompatibility Testing For Cardiovascular Devices in Large Animal Model. J. Vis. Exp. (55), e3197, doi:10.3791/3197 (2011).
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