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Autologous Endothelial Progenitor Cell-Seeding Technology and Biocompatibility Testing For Cardiovascular Devices in Large Animal Model
JoVE 杂志
生物工程
This content is Free Access.
JoVE 杂志 生物工程
Autologous Endothelial Progenitor Cell-Seeding Technology and Biocompatibility Testing For Cardiovascular Devices in Large Animal Model

Autologous Endothelial Progenitor Cell-Seeding Technology and Biocompatibility Testing For Cardiovascular Devices in Large Animal Model

DOI:
10.3791/3197-v

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11:49 min

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September 09, 2011

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, , , , , , , ,
1Department of Biomedical Engineering,Duke University , 2School of Medicine,Duke University , 3Department of Surgery,Duke University Medical Center, 4School of Medicine,University of Pennsylvania

Chapters

  • 00:05Title
  • 02:12Preparing the EPCs for Seeding
  • 04:01Porcine Implantation Surgery: Part 1
  • 06:16Seeding the Implant
  • 07:26Porcine Implantation Surgery: Part 2
  • 09:39Representative Results
  • 10:29Conclusion

Summary

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A method for seeding titanium blood-contacting biomaterials with autologous cells and testing biocompatibility is described. This method uses endothelial progenitor cells and titanium tubes, seeded within minutes of surgical implantation into porcine venae cavae. This technique is adaptable to many other implantable biomedical devices.

Tags

Autologous Endothelial Progenitor Cell-seeding TechnologyBiocompatibility TestingCardiovascular DevicesLarge Animal ModelImplantable DevicesArtificial MaterialsThromboemboli FormationTi TubesBlood-derived Human Or Porcine Endothelial Progenitor Cells (EPCs)Inferior Vena Cava (IVC)BiocompatibilityProthrombotic EnvironmentBare Metal SurfacesEndothelialize DevicesAntithrombotic FunctionPeripheral BloodMononuclear Cell FractionCultureLaparotomy

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