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Immunologie et infection

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从核衣壳的病毒DNA的制备

Journal JoVE
Immunologie et infection

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Journal JoVE Immunologie et infection

Preparation of Viral DNA from Nucleocapsids

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从核衣壳的病毒DNA的制备

20,556 Views

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12:45 min

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August 16, 2011

DOI:

DOI:

•

12:45 min

August 16, 2011

•

13 Views

Moriah L. Szpara¹, Yolanda R. Tafuri¹, L. W. Enquist¹

¹Department of Molecular Biology,Princeton University

Summary

我们描述了从被感染的细胞中分离高纯度的疱疹病毒衣壳DNA的过程。从溶液中捕获的是最后的DNA浓度和纯度高，使得它非常适合高通量测序，高保真PCR反应，并产生新的病毒重组体转染。

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