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Immunologie et infection

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IGY Teknoloji: Yumurta Sarısı Tavuk Antikorlar çıkarımı Polietilen Glikol (PEG) Yağış

Journal JoVE
Immunologie et infection

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Journal JoVE Immunologie et infection

IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation

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IGY Teknoloji: Yumurta Sarısı Tavuk Antikorlar çıkarımı Polietilen Glikol (PEG) Yağış

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07:26 min

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May 01, 2011

DOI:

DOI:

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07:26 min

May 01, 2011

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52 Views

Diana Pauly¹, Pablo A. Chacana², Esteban G. Calzado³, Björn Brembs⁴, Rüdiger Schade⁵

¹Center for Biological Security,Robert Koch-Institute, ²CICVyA – INTA Castelar,Instituto de Virología, ³Center of Molecular Immunology,Ciudad de la Habana, Cuba, ⁴Department of Biology, Chemistry, Pharmacy, Institute of Biology-Neurobiology,Free University of Berlin, ⁵Institut of Pharmacology,Charité-University Medicine of Berlin

Summary

Bu protokol, polietilen glikol yağış yoluyla, özellikle yumurta sarısı toplam IGY çıkarma açıklar ve, IGY teknolojisi hakkında genel bilgi verir.

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IgY Technology Chicken Antibodies Egg Yolk Polyethylene Glycol (PEG) Precipitation Immunization I.m. Vaccination Gene-Gun Plasmid-immunization Antibody-titres Boost Immunizations Laying Capacity Extraction Of Total IgY Precipitation Procedure Removal Of Lipids Dialysis IgY-extract Storage Purity Of The Extract Total IgY Per Egg Age Of The Laying Hen Total Potential Harvest Of IgY

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