Journal
/
Médecine
/
Isolation of Human Islets from Partially Pancreatectomized Patients
Journal JoVE
Médecine
Author Produced
This content is Free Access.
Journal JoVE Médecine
Isolation of Human Islets from Partially Pancreatectomized Patients

Isolation of Human Islets from Partially Pancreatectomized Patients

DOI:
10.3791/2962-v

•

11:10 min

•

July 30, 2011

•

, , , , , , , ,
1Department of GI-, Thorax- and Vascular Surgery,University Hospital Carl Gustav Carus, University of Technology Dresden, 2Molecular Diabetology,Paul Langerhans Institute Dresden, 3Department of Pathology,University Hospital Carl Gustav Carus, University of Technology Dresden

Chapitres

  • 00:03Titre
  • 00:05Introduction
  • 00:54Surgical Procedure
  • 01:35Pathology
  • 07:01Collection and Culture
  • 09:07Conclusion

Summary

Traduction automatique

Traduction automatique

The supply of type 2 diabetic islets for research is insufficient. Here we share our protocol for isolating islets from patients undergoing partial pancreatectomy. This approach represents a unique venue for obtaining islets from type 2 diabetic and clinically matched non-diabetic subjects in adequate numbers for basic and clinical studies.

Tags

Human IsletsIsolationPartially Pancreatectomized PatientsType 2 DiabetesIslets Of Langerhans MalfunctionLimited AvailabilityOrgan DonorsPartial PancreatectomyPancreatic DiseasesConsentLocal Ethics CommitteeSurgical SpecimensDiagnostic Purposes1000 IsletsEnzyme-containing MediaDigestionSurface AreaRicordi Chamber

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/2962?article_type=v

Staining Protocols for Human Pancreatic Islets
check_url/fr/2962?article_type=v

Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens
check_url/fr/2962?article_type=v

Dynamic Visual Tests to Identify and Quantify Visual Damage and Repair Following Demyelination in Optic Neuritis Patients
check_url/fr/2962?article_type=v

Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples
check_url/fr/2962?article_type=v

Isolation of Cancer Stem Cells From Human Prostate Cancer Samples
check_url/fr/2962?article_type=v

In vitro Method to Observe E-selectin-mediated Interactions Between Prostate Circulating Tumor Cells Derived From Patients and Human Endothelial Cells
check_url/fr/2962?article_type=v

Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets
check_url/fr/2962?article_type=v

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile
check_url/fr/2962?article_type=v

Full-root Aortic Valve Replacement by Stentless Aortic Xenografts in Patients with Small Aortic Roots
check_url/fr/2962?article_type=v

Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae

Read Article