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Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
Journal JoVE
Bio-ingénierie
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Journal JoVE Bio-ingénierie
Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

DOI:
10.3791/2896-v

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08:17 min

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July 04, 2011

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, ,
1Bioengineering Department,Northeastern University, 2Department of Pharmaceutical Sciences,Northeastern University

Chapitres

  • 00:05Titre
  • 01:26Preparing the Optode Solution
  • 01:55Creating Sodium Nanosensors
  • 03:15Determining the Optimal Intracellular Nanosensor Response
  • 04:59Intracellular Injection of Nanosensors
  • 05:40Subcutaneous Injection of Nanosensors
  • 07:03Images of In Vivo and Intracellular Sodium Nanosensors
  • 07:40Conclusion

Summary

Traduction automatique

Traduction automatique

Fluorescent nanoparticles produced in our lab are used for imaging ion concentrations and ion fluxes in biological systems such as cells during signaling and interstitial fluid during physiological homeostasis.

Tags

Fluorescent NanoparticlesIon ConcentrationBiological SystemsIon HomeostasisDehydrationRapid Ion FluxesCellular LevelAction PotentialsSodium RegulationMonitoring Sodium LevelsIntracellular Sodium ProbesCalcium ProbesFluorescent NanosensorsSodium ConcentrationsIn VitroIn VivoIon-selective Optode TechnologyPlasticized Polymeric ParticlesSodium Specific Recognition ElementsPH-sensitive FluorophoresAdditivesMechanism Of ActionChange In FluorescenceReversible SensorsSelective For Sodium Over PotassiumDiameter Of NanoparticlesBiocompatibility

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