Journal
/
生物工程
/
Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
JoVE 杂志
生物工程
This content is Free Access.
JoVE 杂志 生物工程
Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems

DOI:
10.3791/2896-v

•

08:17 min

•

July 04, 2011

•

, ,
1Bioengineering Department,Northeastern University, 2Department of Pharmaceutical Sciences,Northeastern University

Chapters

  • 00:05Title
  • 01:26Preparing the Optode Solution
  • 01:55Creating Sodium Nanosensors
  • 03:15Determining the Optimal Intracellular Nanosensor Response
  • 04:59Intracellular Injection of Nanosensors
  • 05:40Subcutaneous Injection of Nanosensors
  • 07:03Images of In Vivo and Intracellular Sodium Nanosensors
  • 07:40Conclusion

Summary

自动翻译

自动翻译

Fluorescent nanoparticles produced in our lab are used for imaging ion concentrations and ion fluxes in biological systems such as cells during signaling and interstitial fluid during physiological homeostasis.

Tags

Fluorescent NanoparticlesIon ConcentrationBiological SystemsIon HomeostasisDehydrationRapid Ion FluxesCellular LevelAction PotentialsSodium RegulationMonitoring Sodium LevelsIntracellular Sodium ProbesCalcium ProbesFluorescent NanosensorsSodium ConcentrationsIn VitroIn VivoIon-selective Optode TechnologyPlasticized Polymeric ParticlesSodium Specific Recognition ElementsPH-sensitive FluorophoresAdditivesMechanism Of ActionChange In FluorescenceReversible SensorsSelective For Sodium Over PotassiumDiameter Of NanoparticlesBiocompatibility

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Magnetic Resonance Elastography Methodology for the Evaluation of Tissue Engineered Construct Growth

Solid Lipid Nanoparticles (SLNs) for Intracellular Targeting Applications

Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

Production of Metal Nanoparticles by Pulsed Laser-ablation in Liquids: A Tool for Studying the Antibacterial Properties of Nanoparticles

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles

Continuous Measurement of Biological Noise in Escherichia Coli Using Time-lapse Microscopy

Functional Transcranial Doppler Ultrasound for Monitoring Cerebral Blood Flow

Electric and Magnetic Field Devices for Stimulation of Biological Tissues

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems

Read Article