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从原核文化精简纯化的质粒DNA

Journal JoVE
Biologie

This content is Free Access.

Journal JoVE Biologie

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures

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从原核文化精简纯化的质粒DNA

DOI:

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06:53 min

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January 05, 2011

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Laura Pueschel, Hongshan Li, Matthew Hymes

¹Pall Life Sciences

Chapitres

00:05Titre
01:02Lysate Clearance
02:58DNA Binding
04:10DNA Recovery
05:16Quantitative and Qualitative Analyses
06:30Conclusion

Summary

Traduction automatique

此协议是一个成本效益的替代方案，为高效的并行澄清和质粒DNA纯化<em> E。大肠杆菌</em>文化。 AcroPrep发展的过程开始于一个优化的裂解液澄清过滤净化结合能力高的DNA结合滤板的车牌。

Tags

Plasmid DNA Purification AcroPrep Advance Filter Plates Prokaryotic Cultures Bacterial Lysate Clearance DNA Purification Streamlined Process Silica-based Media Vacuum Filtration Centrifuge High-quality DNA OD260/280 Ratios Downstream Applications Sequencing Cloning Manual Processing Semi-automated Processing Fully-automated Processing

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