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Neuroscience

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JoVE Journal Neuroscience
Physiological Recordings of High and Low Output NMJs on the Crayfish Leg Extensor Muscle
 

Physiological Recordings of High and Low Output NMJs on the Crayfish Leg Extensor Muscle

Article DOI: 10.3791/2319-v • 10:01 min • November 17th, 2010
November 17th, 2010 •
*1, *1
1Department of Biology, University of Kentucky
* These authors contributed equally

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This article demonstrates how to conduct electrophysiological recordings of synaptic responses on the extensor muscle in the walking leg of a crayfish and how the nerve terminals are visualized to show the gross morphological differences of high- and low-output nerve terminals.

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Physiological Recordings High Output NMJs Low Output NMJs Crayfish Leg Extensor Muscle Electrophysiological Recordings Synaptic Responses Phasic Motor Neurons Tonic Motor Neurons Nerve Terminals Mitochondria Vital Stain Varicosities Filiform Terminal Synaptic Efficacy Facilitated Responses Quantal Efficacy Synaptic Depression High Frequency Stimulation Macropatch Electrode Muscle Fibers Morphological Differentiation Physiological Differentiation
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