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神经科学
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Physiological Recordings of High and Low Output NMJs on the Crayfish Leg Extensor Muscle
JoVE 杂志
神经科学
This content is Free Access.
JoVE 杂志 神经科学
Physiological Recordings of High and Low Output NMJs on the Crayfish Leg Extensor Muscle

Physiological Recordings of High and Low Output NMJs on the Crayfish Leg Extensor Muscle

DOI:
10.3791/2319-v

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10:00 min

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November 17, 2010

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1Department of Biology,University of Kentucky

Chapters

  • 00:00Title
  • 01:28Preparation of the Crayfish Leg Extensor Muscle and Nerves
  • 04:14Nerve Stimulation and Intracellular and Extracellular Recording
  • 07:24Results: Synaptic Facilitation, Synaptic Depression, and Quantal Postsynaptic Potentials
  • 09:38Conclusion

Summary

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This article demonstrates how to conduct electrophysiological recordings of synaptic responses on the extensor muscle in the walking leg of a crayfish and how the nerve terminals are visualized to show the gross morphological differences of high- and low-output nerve terminals.

Tags

Physiological RecordingsHigh Output NMJsLow Output NMJsCrayfish Leg Extensor MuscleElectrophysiological RecordingsSynaptic ResponsesPhasic Motor NeuronsTonic Motor NeuronsNerve TerminalsMitochondriaVital StainVaricositiesFiliform TerminalSynaptic EfficacyFacilitated ResponsesQuantal EfficacySynaptic DepressionHigh Frequency StimulationMacropatch ElectrodeMuscle FibersMorphological DifferentiationPhysiological Differentiation

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