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Neuroscience

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JoVE Journal Neuroscience
Laser Capture Microdissection of Drosophila Peripheral Neurons
 
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Laser Capture Microdissection of Drosophila Peripheral Neurons

Article DOI: 10.3791/2016-v • 12:02 min • May 24th, 2010
May 24th, 2010 •
1,2, 1,2
1Department of Molecular and Microbiology, George Mason University, 2Krasnow Institute for Advanced Study, George Mason University

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In this video-article we present a method for isolating single or multiple Drosophila da neurons from third instar larvae using the infrared capture (IR) class of Laser Capture Microdissection (LCM). RNA obtained from the isolated neurons can be readily used for downstream applications including qRT-PCR or microarray analyses.

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Laser Capture Microdissection Drosophila Peripheral Neurons Dendritic Arborization Da Neurons Molecular Mechanisms Class-specific Dendrite Morphogenesis RNA Isolation Techniques Magnetic Bead-based Cell Purification Fluorescent Activated Cell Sorting (FACS) RNA Binding Protein-based Strategies Laser Capture Microdissection (LCM) Spatial Precision QRT-PCR Microarray Expression Profiling Third Instar Larval Stage
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